HEPES
N-[2-hydroxyethyl] piperazine-N'-[2-ethanesulfonic acid]
Specifications
| CAS | 7365-45-9 |
| Grade | ultra pure |
| Formula weight | 238.31 |
| Formula | C8H18N2O4S |
| Physical description | white crystalline powder |
| Unit | Min | Max | |
|---|---|---|---|
| Appearance (White Crystalline Powder) | Pass | 0 | 0 |
| Identification (IR) | Pass | 0 | 0 |
| Iron | ppm | 0 | 5 |
| Lead (Pb) | ppm | 0 | 5 |
| Protease (None Detected) | Pass | 0 | 0 |
| Purity (By NaOH Titration) | % | 99.5 | 100 |
| Rnase (None Detected) | Pass | 0 | 0 |
| Uv Absorbance At 280nm | nm | 0 | 0.05 |
| Uv Absorbance At 290nm (33% W/w) | nm | 0 | 0.05 |
| Uv Absorbance At 260nm | nm | 0 | 0.05 |
| Water (Kf) | % | 0 | 0.1 |
| Cell Culture Test | Pass | 0 | 0 |
| DNAse, Exonuclease Detection | Pass | 0 | 0 |
| Endotoxin Level (<0.1 Eu/mg) | Pass | 0 | 0 |
| Expiration Verification (1 Yr. Minimum) | Pass | 0 | 0 |
| Nickase, Endonuclease Detection | Pass | 0 | 0 |
| Solubility (Clear, Colorless) | Pass | 0 | 0 |
| Total Aerobic Microbial Count <100CFU/g | Pass | 0 | 0 |
Notes & Applications
biological buffer
pKa (25°C): 7.55 ± 0.2
store at 15°C to 35°C