Sequel™ NE reagent (Part A)
concentrate for sequel™ DNA sequencing solution
Specifications
| 20% acrylamide/bisacrylamide (19:1) | |
| 8m urea | |
| 0.1m tris | |
| 0.08m borate | |
| 1mm edta | |
| 0.075% temed |
| Unit | Min | Max | |
|---|---|---|---|
| Polymerization Test (Sop-0088) | Pass | 0 | 0 |
| Visual Inspection | Pass | 0 | 0 |
Notes & Applications
store at 2°C to 8°C
add ammonium persulfate to initiate polymerization, then cast gel
make any percentage sequencing gel (up to 20%).
Part A contains 20% acrylamide and is mixed with Sequel NE part B to
Sequel™ NE part A is one component of a two part sequencing solution.
for DNA sequence gel